Essential Genetics: A Genomics Perspective, Fourth Edition: Practice Quizzes

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Title Text: Essential Genetics: A Genomics Perspective, Fourth Edition, Daniel L. Hartl and Elizabeth W. Jones

Please read each question and select your answer from the choices provided. You must complete all of the questions in order to view your results. At the end of each exam, you have the option to e-mail your results to your instructor.

1: The cloning site containg unique cleavage sites for many different restriction enzymes is called a(an)
	A: Sequence-tagged site
	B: Vector
	C: Contig
	D: Polylinker
	E: Polysome

2: The procedure used to determine experimentally the physical limits of a gene is called
	A: Cotransformation
	B: Targeted mutagenesis
	C: Hybridisation with a labeled probe
	D: Yeast two-hybrid system
	E: Transformation resque

3: A DNA molecule has 28 occurrences of the sequence 5'-GGCC-3' along one strand. How many times does the same sequence occur along the other strand?
	A: 7
	B: 14
	C: 21
	D: 28
	E: 32

4: A number of restriction enzymes cleave both DNA strands at the center of symmetry, forming
	A: Cohesive ends
	B: Blunt ends
	C: Sequence-tagged sites
	D: Contigs
	E: Polylinker

5: You digested a linear DNA molecule with the restriction enzyme and got 3 fragments. How many restriction sites does this DNA have for this particular endonuclease?
	A: 4
	B: 3
	C: 2
	D: 1
	E: 0

6: Deliberate alteration of the genome for treatment of disease is called
	A: Transformation resque
	B: Gene therapy
	C: Enhancer trap
	D: Exon shuffle
	E: Imprinting

7: Term for the double-stranded DNA produced by reverse transcriptase is
	A: cDNA
	B: Cosmid
	C: STS
	D: RLFP
	E: Ti DNA

8: DNA produced by reverse transcriptase11. ________________________Use of a transgenic organism
	A: 6
	B: 12
	C: 120
	D: 1200

9: In gene-targeting experiments, the replacement of the wildtype gene in the genome with the completely unfunctional gene results in a (n)
	A: Imprinting
	B: Transformation resque
	C: Enhancer trap
	D: DNA looping
	E: Knockout mutation

10: Loss of b-galactosidase activity is often used to detect
	A: DNA hairpins
	B: Single-stranded DNA
	C: Introns
	D: Recombinant vectors
	E: Polylinkers

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