Brief Table of Contents
Foreword by Elaine Mardis
Chapter 1: Controlled Heat-Denaturation of DNA Plasmids
Jan Kieleczawa, Wyeth Research
Chapter 2: Effect of Various Reagents on DNA Sequencing
Jan Kieleczawa, Wyeth Research
Chapter 3: Sequencing of Difficult DNA Templates
Jan Kieleczawa, Wyeth Research
Sandra L. Spurgeon and John W. Brandis, Applied Biosystems
Chapter 5: Beyond pUC: Vectors for Cloning Unstable DNA
Ronald Godiska, Melodee Patterson, Tom Schoenfeld, and David A. Mead, Lucigen Corporation, Middleton, WI
Vincent Ling, Wyeth Research
Chapter 7: Plasmid Preparation Methods for DNA Sequencing
Chapter 8: Optimization of Culture Growth in 96-Deep-Well Plates
Jan Kieleczawa, Wyeth Research
Jan Kieleczawa, Wyeth Research
Chapter 10: Geospiza’s Finch-Server: A Complete Data Management System for DNA Sequencing
Sandra Porter, Joe Slagel, and Todd Smith, Geospiza, Inc.
Chapter 11: DNA Sequencing Database: A Flexible LIMS for DNA Sequencing Analysis
Donald M. Koffman, DMK Concepts Inc. and Hemchand Sookdeo, Wyeth Research
Chapter 12: Good Laboratory Practices, Good Manufacturing Procedures, and Quality Assurance in the DNA Sequencing Laboratory
Michele Godlevski and Thalia Taylor, GlaxoSmithKline
Chapter 13: The Future of DNA Sequencing: Towards an Affordable Genome
Kevin McKernan, Agencourt Biosciences
